T4 Polynucleotide Kinase

Catalogue Number: MB00801-NZY

Manufacturer:	NZYTech
Type:	Kinases
Shipping Condition:	Dry Ice
Storage Condition:	-70°C
Unit(s):	500 U

Overview

Description

Description: T4 Polynucleotide Kinase (T4 PNK) catalyses the transfer of the terminal phosphate of ATP to 5'-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3'-mononucleotides. This enzyme also possesses a 3'-phosphatase and 2', 3' cyclic phosphodiesterase activity. The enzyme can be used for the following applications: 5'-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation; end-labelling DNA or RNA for use as probes, for DNA sequencing or for DNA-protein foot printing; removal of 3' phosphoryl groups. Applications: - End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting - 5'-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation - Removal of 3'-phosphoryl groups

Additional Text

Short Description

T4 Polynucleotide Kinase catalyses the transfer of the terminal phosphate of ATP to 5'-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3'-mononucleotides.

Price

£48.00

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