Klenow Fragment of DNA Polymerase I

Catalogue Number: MB00901-NZY

Manufacturer:	NZYTech
Type:	DNA Polymerases
Shipping Condition:	Dry Ice
Storage Condition:	-70°C
Unit(s):	300 U

Overview

Description

Description: Klenow Fragment of DNA Polymerase I is a truncated fragment of E. coli DNA polymerase I that lacks the 5' to 3' exonuclease activity while retaining the 3' to 5' exonuclease activity (proofreading activity). The enzyme is well suited for use in 3'-end labelling of DNA fragments for sequence analysis, for the creation of bluntends by the filling-in of 5'-overhangs or by the removal of 3'-overhangs, for second-strand synthesis of cDNA and for random primer labelling of DNA fragments. Applications: - 3'-end labelling of DNA fragments for sequence analysis - Conversion of 5'-overhangs to create blunt-ends for cloning - Removal of 3'-overhangs - Second-strand synthesis of cDNA - Random primer labelling of DNA fragments

Additional Text

Short Description

Klenow Fragment of DNA Polymerase I is a truncated fragment of E. coli DNA polymerase I that lacks the 5' to 3' exonuclease activity while retaining the 3' to 5' exonuclease activity (proofreading).

Price

£48.00

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