Catalogue Number: MB08001-NZY
Manufacturer: | NZYTech |
Type: | RNA polymerases |
Shipping Condition: | Dry Ice |
Storage Condition: | -70°C |
Unit(s): | 10000 units |
Description: T7 RNA polymerase is a recombinant enzyme purified from Escherichia coli, for the synthesis of high specific-activity RNA probes, biologically active mRNA and antisense RNA. T7 RNA polymerase is DNA-dependent with strict specificity for its own double-stranded promoter that is not efficiently recognized by SP6 or T3 RNA polymerases. T7 RNA polymerase catalyzes the 5' to 3' synthesis of RNA from ribonucleoside triphosphates on single or double-stranded DNA downstream from a T7 promoter. Using circular plasmid DNA as a template will result in heterogeneous transcripts of multiple lengths. T7 RNA polymerase accepts modified nucleotides as substrates for RNA synthesis.
T7 RNA Polymerase is a DNA-dependent RNA polymerase with high promoter-specificity. The enzyme only transcribes DNA downstream of a T7 promoter.