NZY RNase H (E. coli)
Catalogue Number: MB08501-NZY
| Manufacturer: | NZYTech |
| Type: | Ribonucleases (RNase) |
| Shipping Condition: | Dry Ice |
| Storage Condition: | -70°C |
| Unit(s): | 250 U |
Description
Description: NZY RNase H is a recombinant endoribonuclease purified from an Escherichia coli strain that over-expresses the cloned RNase H gene (rnh). The enzyme specifically hydrolyses the phosphodiester bonds of RNA which is hybridized to DNA to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA. RNase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as cDNA-RNA hybrids in the amplification reaction may prevent binding of the amplification primers. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets.
Additional Text
Short Description
NZY RNase H is a key enzyme in the synthesis of cDNA. It specifically hydrolyses RNA in RNA:cDNA complexes, thus improving sensitivity. The enzyme does not degrade single and double-stranded DNA or unhybridized RNA.
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