NZY First-Strand cDNA Synthesis Kit

Catalogue Number: MB12501-NZY

Manufacturer:	NZYTech
Type:	cDNA Synthesis Kits
Shipping Condition:	Dry Ice
Storage Condition:	-70°C
Unit(s):	50 reactions
Application:	cDNASyn

Overview

Description

Description: NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2x Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA.

Additional Text

Short Description

NZY First-Strand cDNA Synthesis Kit is a convenient and reliable system that includes all the necessary components to synthesize first-strand cDNA. The kit allows reaction at high temperatures, which are benefic when starting from difficult and complex RNA.

Price

£111.00

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