NZY-A Speedy PCR cloning kit
Catalogue Number: MB13701-NZY
| Manufacturer: | NZYTech |
| Type: | DNA Cloning Kits |
| Shipping Condition: | Dry Ice |
| Storage Condition: | -70°C |
| Unit(s): | 24 ligations |
| Application: | PCR, Cln |
Description
Description: NZY-A Speedy PCR cloning kit was designed to carry out fast and efficient cloning of PCR products with 3'-A overhangs, which result from amplifications using non-proofreading DNA polymerases such as NZYTaq II DNA polymerase. This methodology combines the efficiency of an improved ligation buffer with the speed of Speedy Ligase to allow a rapid ligation between the vector and the PCR product in only 5 minutes at room temperature (18-25 °C). The cloning vector was prepared by cutting NZYTech's pNZY28 with EcoR V and adding a 3' terminal thymidine at both ends. These single 3'-T overhangs improve the efficiency of ligation of a PCR product into plasmids by preventing re-circularization of the vector and providing compatible overhangs for PCR products generated by non-proofreading thermostable polymerases. Vector pNZY28 contains multiple restriction sites within the multiple cloning region. However, vector digestion with EcoR I or BamH I allows the release of the PCR product since the vector cloning region is flanked by recognition sites of both enzymes.
Additional Text
Short Description
NZY-A Speedy PCR cloning kit was designed to carry out fast and efficient cloning of PCR products with 3'-A overhangs, which result from amplifications using non-proofreading DNA polymerases.
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations