NZY-A Speedy PCR cloning kit

Catalogue Number: MB13702-NZY

Manufacturer:	NZYTech
Type:	DNA Cloning Kits
Shipping Condition:	Dry Ice
Storage Condition:	-70°C
Unit(s):	24 ligations
Application:	PCR, Cln

Overview

Description

Description: NZY-A Speedy PCR cloning kit was designed to carry out fast and efficient cloning of PCR products with 3'-A overhangs, which result from amplifications using non-proofreading DNA polymerases such as NZYTaq II DNA polymerase. This methodology combines the efficiency of an improved ligation buffer with the speed of Speedy Ligase to allow a rapid ligation between the vector and the PCR product in only 5 minutes at room temperature (18-25 °C). The cloning vector was prepared by cutting NZYTech's pNZY28 with EcoR V and adding a 3' terminal thymidine at both ends. These single 3'-T overhangs improve the efficiency of ligation of a PCR product into plasmids by preventing re-circularization of the vector and providing compatible overhangs for PCR products generated by non-proofreading thermostable polymerases. Vector pNZY28 contains multiple restriction sites within the multiple cloning region. However, vector digestion with EcoR I or BamH I allows the release of the PCR product since the vector cloning region is flanked by recognition sites of both enzymes.

Additional Text

Short Description

NZY-A Speedy PCR cloning kit was designed to carry out fast and efficient cloning of PCR products with 3'-A overhangs, which result from amplifications using non-proofreading DNA polymerases.

Price

£98.00

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