DpnII
Catalogue Number: MB23302-NZY
| Manufacturer: | NZYTech |
| Type: | Restriction Enzymes |
| Shipping Condition: | Dry Ice |
| Unit(s): | 5000 U |
Description
Description: Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. DpnII is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnII gene from Diplococcus pneumoniae. DpnII activity is blocked by Dam methylation. The optimum reaction temperature is at 37 °C. Concentration: 10000 U/mL Reaction Buffer: NZYTech provides DpnII with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided). Activity in NZYTech Buffers: NZYBuffer A: 80% NZYBuffer B: 80% NZYBuffer C: 100% NZYBuffer U: 100% Minimize exposure of enzyme to temperatures higher than -20 °C.
Additional Text
Purification
Purified
Short Description
DpnII is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnII gene from Diplococcus pneumoniae. DpnII activity is blocked by Dam methylation. NZYTech's DpnII is an enzyme that acts at 37 °C.
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