DdeI
Catalogue Number: MB23601-NZY
| Manufacturer: | NZYTech |
| Type: | Restriction Enzymes |
| Shipping Condition: | Dry Ice |
| Unit(s): | 500 U |
Description
Description: Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. DdeI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DdeI gene from Desulfovibrio desulfuricans. DdeI activity is not affected by Dam methylation, Dcm methylation or CpG methylation. The optimum reaction temperature is at 37 °C. Concentration: 10000 U/mL Reaction Buffer: NZYBuffer U, which allows double digestions and the direct load of the restriction digests onto agarose gels. Activity in NZYTech Buffers: NZYBuffer A: 25% NZYBuffer B: 25% NZYBuffer C: 25% NZYBuffer U: 100% Minimize exposure of enzyme to temperatures higher than -20 °C.
Additional Text
Purification
Purified
Short Description
DdeI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DdeI gene from Desulfovibrio desulfuricans. DdeI activity is not affected by Dam methylation, Dcm methylation or CpG methylation.
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