TBE (Tris-Borate-EDTA) Buffer 10x, pH 8.3 (liquid)
Catalogue Number: MB27701-NZY
| Manufacturer: | NZYTech |
| Type: | Biological Buffers |
| Shipping Condition: | RT |
| Storage Condition: | RT |
| Unit(s): | 1000 ml |
| Application: | PCR, Elctrphs |
Description
Description: Specifications: -Tris 0.9 M 108 g/L -Boric acid 0.9 M 55 g/L -Na2EDTA 0.02 M 43.5 g/L Directions for use: Dilute 100 mL of TBE Buffer, 10x stock solution into 900 mL deionised water to make 1 litre of TBE Buffer. Final concentration is 89 mM Tris base, 89 mM Borate and 2 mM Na2EDTA. On dilution to 1X the user should check pH and adjust as required.
Additional Text
Short Description
In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE (Tris-Borate-Acetate) buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments.
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