Supreme NZYTaq II DNA Polymerase with 5x Gel Load

Catalogue Number: MB36503-NZY

Manufacturer:	NZYTech
Type:	DNA Polymerases
Shipping Condition:	Blue Ice
Storage Condition:	-70°C
Unit(s):	2500 units
Application:	PCR

Overview

Description

Description: Supreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity. The enzyme activity at room temperature is limited, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity, sensitivity and yield during DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. Supreme NZYTaq II is provided with 5x Gel Load Reaction Buffer allowing reactions to be loaded directly into gels without the extra adding of loading dye. This Gel Load Reaction Buffer is composed by a blue and yellow dye. The blue dye migrates at the same rate as a 3-5 kb DNA fragment in a 1% (w/v) agarose gel. The yellow dye migrates at a rate faster than primers (< 50 bp) in a 1% (v/v) agarose gel. The 5x Gel Load Reaction Buffer is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. Supreme NZYTaq II DNA polymerase lacks 3' to 5' exonuclease activity. Resulting PCR products have an A overhang and are suitable for cloning with NZYTech's TA PCR cloning kits (MB053 or MB137).

Additional Text

Short Description

Supreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity.

Price

£232.00

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