Catalogue Number: MB41402-NZY
Manufacturer: | NZYTech |
Type: | qPCR Mixes |
Shipping Condition: | Blue Ice |
Storage Condition: | -70°C |
Unit(s): | 5 x 1 ml |
Application: | RT-PCR, cDNASyn, RT-qPCR |
Description: NZYSupreme One-step RT-qPCR Probe Master Mix is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimizes the risk of errors and contaminations. The master mix, formulated as a 2x reaction mixture, contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology. For qPCR instruments that require this reference dye, we indicate a concentration range for your assay according to the table shown in the section "ROX reference dye" (ROX not provided).
NZYSupreme One-step RT-qPCR Probe Master Mix is a highly efficient and optimized reaction mixture that enables convenient and reliable first-strand cDNA synthesis and real-time PCR in one tube.