DNA Polymerase I (E.coli)

Catalogue Number: MB42001-NZY

Manufacturer:	NZYTech
Type:	DNA Polymerases
Shipping Condition:	Dry Ice
Storage Condition:	-70°C
Unit(s):	500 U

Overview

Description

Description: DNA Polymerase I (E. coli) is a non-thermostable DNA polymerase with inherent 3' to 5' (proofreading) and 5' to 3' exonuclease activities, in addition to a lower and non-specific ribonuclease H activity. The 5' to 3' exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation. Thus, DNA Polymerase I (E. coli) displays no strand-displacement activity and may be used for DNA labelling by nick-translation, in conjunction with DNase I, or second-strand cDNA synthesis, in conjunction with RNase H. DNA Polymerase I (E. coli) accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labelled nucleotides) as substrates for the DNA synthesis. Applications: - DNA labelling by nick-translation - Second-strand synthesis of cDNA

Additional Text

Short Description

DNA Polymerase I (E. coli) is a non-thermostable DNA polymerase with inherent 3' to 5' (proofreading) and 5' to 3' exonuclease activities, in addition to a lower and non-specific ribonuclease H activity.

Price

£39.00

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