Catalogue Number: MB42701-NZY
Manufacturer: | NZYTech |
Type: | Ligases |
Shipping Condition: | Dry Ice |
Storage Condition: | -70°C |
Unit(s): | 1000 U |
Description: T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Note that the minimal substrate for T4 ssRNA Ligase is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8 bases in intramolecular reaction. The enzyme may be used for the ligation of ssRNA and DNA, 3'-termini of RNA labelling, synthesis of single-stranded oligoribo- and oligodeoxyribonucleotides and specific modifications of tRNAs. Applications: - Ligation of ssRNA and DNA - 3'-end labelling of single strand RNA - Synthesis of single-stranded oligoribonucleotides and oligodeoxyribonucleotides - Specific modifications of tRNAs
T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.