T4 ssRNA Ligase

Catalogue Number: MB42701-NZY

Manufacturer:NZYTech
Type:Ligases
Shipping Condition:Dry Ice
Storage Condition:-70°C
Unit(s): 1000 U

Description

Description: T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Note that the minimal substrate for T4 ssRNA Ligase is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8 bases in intramolecular reaction. The enzyme may be used for the ligation of ssRNA and DNA, 3'-termini of RNA labelling, synthesis of single-stranded oligoribo- and oligodeoxyribonucleotides and specific modifications of tRNAs. Applications: - Ligation of ssRNA and DNA - 3'-end labelling of single strand RNA - Synthesis of single-stranded oligoribonucleotides and oligodeoxyribonucleotides - Specific modifications of tRNAs

Additional Text

Short Description

T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.