Description

Description: NZYTech's COVID-19 & Flu A/B Multiplex One-Step RT-qPCR Kit, IVD provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 and/or Influenza genomes, through common real-time PCR platforms. SARS-CoV-2 is identified by detecting RT-qPCR targets located in RdRp and N genes. In contrast, Influenza A and Influenza B viruses are detected through the amplification of targets located in M1 and NS2 genes, respectively. NZYTech's COVID-19 & Flu A/B Multiplex One-Step RT-qPCR Kit, IVD is built to have the broadest detection profile possible whilst remaining specific to the SARS-CoV-2 and Influenza types A and B genomes. NZYTech's COVID-19 & Flu A/B Multiplex One-Step RT-qPCR Kit, IVD is a multiplex assay detecting SARS-CoV-2, Influenza A, Influenza B and Human nucleic acids (acting as an internal positive control). Extracted and purified RNA is transcribed to cDNA and subsequently amplified in a single reaction using five highly specific primers/probe sets, namely those detecting SARS-CoV-2 RNA dependent RNA polymerase (RdRp) and the Nucleocapsid phosphoprotein (N) genes, Influenza A and B Matrix (M1) and Nonstructural 2 (NS2) specific genes and the human ribonuclease P (RNase P, RP) gene. The kit exploits the so-called TaqMan® principle. During this process, probes specifically anneal to their target genes and upon DNA amplification, through two flanking primers, are subjected to degradation leading to the separation of the reporter dye from the quencher thus resulting in an increase in fluorescence. Detection of the internal control (the human RNase P gene) validates the efficacy of the extraction process as well as the absence of PCR inhibitors potentially present in the human biological samples. To allow identifying the amplification of the five specific targets in a single reaction, SARS-CoV-2, Influenza A/B and human RNase P specific probes are differently labelled, with Texas Red®, FAM™ and HEX™ reporter dyes, respectively. Note that this panel contains a duplex assay in the Texas Red® (SARS-CoV-2 RdRp and N specific target genes) and FAM™ (Influenza A and Influenza B specific target genes) channels. This allows reporting an additive performance of assays for SARS-CoV-2 detection but precludes distinguishing between Influenza A/B infections. In addition, the five primers/probe sets are provided in optimized concentrations guaranteeing that amplification of lower abundant nucleic acids is not compromised when other viral targets are present at higher concentrations. In case the IFU is not available in your official country language, please contact us - info@nzytech.com so that we can proceed with the translation. Please see languages available below. Find the IFU for your product batch

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Short Description

NZYTech's COVID-19 & Flu A/B Multiplex One-Step RT-qPCR Kit, IVD provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 and/or Influenza genomes, through common real-time PCR platforms.