SARS-CoV-2 One-Step RT-PCR Kit II, RdRp and N Genes

Catalogue Number: MD04871-NZY

Manufacturer:	NZYTech
Type:	PCR Kits
Shipping Condition:	Blue Ice
Storage Condition:	-70°C
Unit(s):	96 reactions
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Application:	RT-PCR

Overview

Description

Description: NZYTech SARS-CoV-2 One-Step RT-PCR Kit II, RdRp and N genes, IVD provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 genome, through common real-time PCR platforms. The virus RNA dependent RNA polymerase (RdRp) and the Nucleocapsid phosphoprotein (N) genes have previously been identified as highly specific markers for SARS-CoV-2. This NZYTech kit targets specific regions in the RdRp and N genes of SARS-CoV-2 genome to provide the highest sensitivity of detection. By using NZYTech SARS-CoV-2 One-Step RT-PCR Kit II, RdRp and N genes, IVD, RNA isolated and purified with a IVD extration system is retrotranscribed (RT) to cDNA and subsequently amplified by PCR, in a single reaction, using three highly specific primer/probe sets exploiting the so-called TaqMan® principle. During this process, the probes specifically anneal to two regions of the SARS-CoV-2 genome, namely RdRp (within the Orf1ab polyprotein gene) and N genes, in case the sample was extracted from an infected patient. An additional primers/probe set acts as an endogenous internal control to detect nucleic acids of the human ribonuclease P [RNase P gene (RP)], assessing sample quality. In addition, this internal control demonstrates that no reaction inhibition has occurred by PCR inhibitors potentially present in the clinical/environmental samples. To allow identifying the amplification of the three specific targets in a single reaction, SARS-CoV-2 (RdRp and N genes) and human RNase P specific probes are differently labelled, with HEX™, FAM™ and Cy5™ reporter dyes, respectively. Note that this panel contains a duplex assay in two distinct optical channels - HEX™ and FAM™ - to report performance of the two PCR assays for SARS-CoV-2 detection. In addition, they are provided in optimized concentrations to make sure amplification of human mRNA, even when present at very high concentrations, does not limit the efficiency of the SARS-CoV-2 primers/probe sets. In case the IFU is not available in your official country language, please contact us - info@nzytech.com so that we can proceed with the translation. Please see languages available below. Find the IFU for your product batch

Additional Text

Short Description

NZYTech SARS-CoV-2 One-Step RT-PCR Kit II, RdRp and N genes, IVD provides the complete set of reagents and probes to qualitatively detect the SARS-CoV-2 genome, through common real-time PCR platforms.

Price

£305.00

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