Catalogue Number: MD05291-NZY
Manufacturer: | NZYTech |
Type: | qPCR Kits |
Shipping Condition: | RT |
Storage Condition: | -70°C |
Unit(s): | 100 reactions |
Application: | qPCR |
Description: Lyme disease is a vector-borne disease caused by the spirochete bacterium Borrelia. This bacterium is transmitted to humans by the bites of infected ticks of the genus Ixodes. Lyme Disease qPCR Kit is designed for the in vitro detection of Borrelia species genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Borrelia spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with a broad range of Borrelia spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. This kit detects the following Borrelia species that are the most significant causative agents of Lyme disease: B. burgdorferi, B. garinii and B. afzelii. Other Borrelia species and other closely related genus are not detected. Real-time PCR detection methods have been used in several studies to identify Borrelia spp. genomes (Saidac D.S. et al 2009). If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Lyme Disease qPCR Kit is designed for the in vitro detection of Borrelia spp. genomes.