Catalogue Number: MD06071-NZY
Manufacturer: | NZYTech |
Type: | qPCR Kits |
Shipping Condition: | RT |
Storage Condition: | -70°C |
Unit(s): | 100 reactions |
Application: | qPCR |
Description: Brachyspira pilosicoli is a gram-negative, anaerobic, host-associated spirochete that colonizes the intestinal tract of animals and humans. It is the causative agent of intestinal spirochetosis in pigs, chickens and humans. In particular, B. pilosicoli has been described as an important colonic pathogen of pigs and chickens, causing colitis and diarrhea resulting in depressed rates of growth and impaired production on farms where infections with B. pilosicoli may be endemic. Brachyspira pilosicoli qPCR Kit is designed for the in vitro detection of B. pilosicoli genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to B. pilosicoli. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of B. pilosicoli genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Brachyspira pilosicoli qPCR Kit is designed for the in vitro detection of B. pilosicoli genomes.