Catalogue Number: MD06141-NZY
Manufacturer: | NZYTech |
Type: | qPCR Kits |
Shipping Condition: | RT |
Storage Condition: | -70°C |
Unit(s): | 100 reactions |
Application: | RT-qPCR |
Description: Encephalomyocarditis virus (EMCV) is a positive single-stranded RNA virus that belongs to the genus Cardiovirus within the family Picornaviridae. EMCV is the causative agent of encephalomyocarditis (EMC) in a wide range of vertebrates. Despite being regarded as a rodent virus, it can infect a wide range of mammals, including non-human primates, elephants, pigs and humans. Outbreaks of EMC have a higher incidence in pigs and wildlife species held in captivity. Infected pigs typically present cardiac insufficiency and cardiogenic pulmonary oedema, with a higher mortality in suckling pigs. Encephalomyocarditis virus RT-qPCR Kit is designed for the in vitro detection of EMCV. The kit is built to have the broadest possible detection profile whilst remaining specific to EMCV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of EMCV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Encephalomyocarditis virus RT-qPCR Kit is designed for the in vitro detection of EMCV genomes.