Catalogue Number: MD06221-NZY
Manufacturer: | NZYTech |
Type: | qPCR Kits |
Shipping Condition: | RT |
Storage Condition: | -70°C |
Unit(s): | 100 reactions |
Application: | qPCR |
Description: Dichelobacter nodosus and Fusobacter necrophorum are two Gram-negative, nonmotile and anaerobic bacteria. These two bacteria can work synergistically causing footrot (infectious pododermatitis), a hoof infection commonly found in sheep, goats and cattle. It is responsible for the majority of cases of lameness in sheep. Dichelobacter nodosus is the main cause of the disease, while Fusobacterium necrophorum is an associated pathogen. Dichelobacter nodosus & Fusobacter necrophorum qPCR Kit is designed for the in vitro detection of D. nodosus and F. necrophorum subsp. necrophorum genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to these species. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of D. nodosus and F. necrophorum genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYTech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Dichelobacter nodosus & Fusobacter necrophorum qPCR Kit is designed for the in vitro detection of D. nodosus and F. necrophorum genomes.