Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein (MHC-HM406T)
Catalogue Number: MHC-HM406T-KAT
MHC-HM406T Image
MHC-HM406T Image
MHC-HM406T Image
MHC-HM406T Image
MHC-HM406T Image
Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200) (QC Test).
MHC-HM406T Image
Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 1.65 µM as determined in SPR assay (Biacore T200).
MHC-HM406T Image
The purity of Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 95% as determined by SEC-HPLC.
MHC-HM406T Image
Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Alias: | HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | WB |
Description
Description: HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
Additional Text
Protein Tag
HIS-AviTag™
Short Description
Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Gene Name
B2M, HLA-E
Uniprot ID
P13747, P61769
Gene ID
3133; 567
MHC-HM406T Datasheet
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