Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein (MHC-HM418B)
Catalogue Number: MHC-HM418B-KAT
MHC-HM418B Image
MHC-HM418B Image
MHC-HM418B Image
MHC-HM418B Image
MHC-HM418B Image
Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody with an affinity constant of 0.14 µM as determined in SPR assay (Biacore T200).
MHC-HM418B Image
Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag at 1µg/ml (100µl/well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.29µg/ml determined by ELISA (QC Test).
MHC-HM418B Image
The purity of Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.
MHC-HM418B Image
Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Additional Text
Gene Name
B2M
Gene ID
567
Uniprot ID
P61769
Accession Number
NP_002107.3
Protein Tag
HIS-AviTag™
Short Description
Recombinant Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
MHC-HM418B Datasheet
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