Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein (MHC-HM431T)
Catalogue Number: MHC-HM431T-KAT
MHC-HM431T Image
MHC-HM431T Image
MHC-HM431T Image
MHC-HM431T Image
MHC-HM431T Image
Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody captured on Protein A chip, can bind Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag with an affinity constant of 0.18nM as determined in a SPR assay (Biacore T200).
MHC-HM431T Image
Immobilized Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA (QC Test).
MHC-HM431T Image
The purity of Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer was greater than 95% as determined by SEC-HPLC.
MHC-HM431T Image
Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Alias: | MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33 |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
Additional Text
Gene Name
B2M
Gene ID
567
Protein Tag
HIS-AviTag™
Short Description
Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Uniprot ID
A0A140T913, P61769
MHC-HM431T Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations