Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein (MHC-HM454)
Catalogue Number: MHC-HM454-KAT
MHC-HM454 Image
MHC-HM454 Image
MHC-HM454 Image
MHC-HM454 Image
MHC-HM454 Image
Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag captured on CM5 Chip via anti-his antibody can bind HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR with an affinity constant of 6.93 nM as determined in SPR assay (Biacore T200).
MHC-HM454 Image
Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 5µg/ml (100µl/well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 66.0ng/ml determined by ELISA (QC Test).
MHC-HM454 Image
The purity of HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer is greater than 95% as determined by SEC-HPLC.
MHC-HM454 Image
HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Host Cell: | E.coli |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Additional Text
Gene Name
B2M
Gene ID
567
Uniprot ID
P61769
Protein Tag
HIS-AviTag™
Accession Number
AAV53343.1
Short Description
Recombinant Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein is expressed from E.coli with with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVVGADGVGK peptide.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
MHC-HM454 Datasheet
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