Human MICA Protein (MIC-HM20A)
Catalogue Number: MIC-HM20A-KAT
MIC-HM20A Image
MIC-HM20A Image
MIC-HM20A Image
MIC-HM20A Image
MIC-HM20A Image
MIC-HM20A Image
Human MICA, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.36 µM as determined in SPR assay (Biacore T200).
MIC-HM20A Image
Immobilized Human MICA, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-MICA Antibody, hFc Tag with the EC50 of 0.45µg/ml determined by ELISA (QC Test).
MIC-HM20A Image
Immobilized Human NKG2D, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 33.4ng/ml determined by ELISA.
MIC-HM20A Image
The purity of Human MICA is greater than 95% as determined by SEC-HPLC.
MIC-HM20A Image
Human MICA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 59.64 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Fusion Protein |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
Additional Text
Gene Name
MICA
Gene ID
100507436
Uniprot ID
Q96QC4
Protein Tag
hFC Tag
Short Description
Recombinant Human MICA Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu24-Gln308.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
MIC-HM20A Datasheet
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