5-Azido-C3-UTP
Catalogue Number: NU-157S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 ul |
| Application: | Cnjg |
Additional Text
Concentration
10-11 mM
Application Notes
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested). For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5. 3'-Azide-Labeling of T7 promotor-containing oligonucleotides[1,2,3] The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice - to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively) - to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively) - to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins
Short Description
5-(3-Azidopropyl)-uridine-5'-triphosphate, Triethylammonium salt
Smile String
C(CCc1cn(c(=O)[nH]c1=O)[C@H]1[C@@H]([C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N=[N+]=[N-]
Molecular Weight
567.23 g/mol (free acid)
Molecular Formula
C12H20N5O15P3 (free acid)
Storage Note
store at -20 °C|12 months after date of delivery|avoid freeze/thaw cycles|Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-157S Datasheet
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