DIG-11-ddUTP
Catalogue Number: NU-1619-DIGX-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 ul |
Description
Description: Digoxigenin-11-ddUTP is enzymatically incorporated into the 3'-End of oligonucleotides. It acts as a chain terminator leading to the addition of one Digoxigenin label per molecule only. The resulting Digoxigenin-labeled oligonucleotide probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups: 10:1. Please note: The optimal final concentration and molar ratio of Digoxigenin-11-ddUTP to free 3'-OH groups may very depending on the application and assay conditions. For optimal incorporation rates an individual optimization of the Digoxigenin-11-ddUTP concentration and molar ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Short Description
Digoxigenin-3-O-methylcarbonyl-ε-aminocaproyl-[5-(3-aminopropargyl) -2',3'-dideoxyuridine-5'-triphosphate], Triethylammoniumsalt
Smile String
O=C(NCCCCCC(=O)NCC#Cc1c(=O)[nH]c(=O)n([C@@H]2O[C@H](COP(=O)(OP(=O)(OP(=O)(O)O)O)O)CC2)c1)CO[C@H]1CC[C@@]2([C@H]3C[C@@H](O)[C@]4(C)[C@@H](C5=CC(=O)OC5)CC[C@]4(O)[C@@H]3CC[C@@H]2C1)C
Molecular Formula
C43H63N4O20P3 (free acid)
Molecular Weight
1048.91 g/mol (free acid)
NU-1619-DIGX MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations