Azide-PEG4-aminoallyl-dUTP
Catalogue Number: NU-1705L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/- |
| Physical state: | Solution |
| Type: | Nucleotides |
| Shipping Condition: | Blue Ice |
| Storage Condition: | RT |
| Unit(s): | 5 x 50 ul |
Description
Description: Azide-PEG4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasksto introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for subsequent microscopic imagingto crosslink the DNA to Azide- or DBCO-functionalized biomolecules e.g.proteins
Additional Text
Concentration
10-11 mM
Application Notes
Incorporation into DNA/cDNA byPCR with Taq polymerase in-house data3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[1]
Short Description
5-(15-Azido-4,7,10,13-tetraoxa-pentadecanoyl-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Smile String
[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCOCCOCCOCCOCCN=[N+]=[N-])O
Molecular Weight
796.51 g/mol (free acid)
Molecular Formula
C23H39N6O19P3 (free acid)
Storage Note
store at -20 °C|12 months after date of delivery||Short term exposure (up to 1 week cumulative) to ambient temperature possible.
NU-1705L Datasheet
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