pCp-Biotin
Catalogue Number: NU-1706-BIO-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 ul |
Description
Description: pCp-Biotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Biotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Biotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Biotin moiety can be performed using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Biotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into RNA/miRNA by3'-End Labeling with T4 RNA Ligase
Short Description
Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Biotin, Triethylammonium salt
Smile String
Nc1nc(=O)n(cc1)[C@@H]1O[C@H](COP(=O)(O)O)[C@H]([C@H]1O)OP(=O)(OCCCCCCNC(=O)CCCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2)O
Molecular Weight
728.65 g/mol (free acid)
Molecular Formula
C25H42N6O13P2S1 (free acid)
NU-1706-BIO MSDS
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