pCp-Cy3

Catalogue Number: NU-1706-CY3-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Ph:7.5 +/-0
Physical state:Solution
Type:Labelled Nucleotides
Shipping Condition:Blue Ice
Unit(s): 20 ul

Description

Description: pCp-Cy3 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Please note: Protect the Dye-labeled pCp from exposure to light and carry out experimental procedures under low light conditions.

Additional Text

Concentration

1.0-1.1 mM

Application Notes

Incorporation into RNA/miRNA by3'-End Labeling with T4 RNA Ligase[1]

Short Description

Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Cy3, Triethylammonium salt

Smile String

C(=CC=C/1N(c2c(C1(C)C)cc(cc2)S(=O)(=O)O)CCCCCC(=O)NCCCCCCOP(=O)(O[C@@H]1[C@@H](COP(=O)(O)O)O[C@@H](n2c(=O)nc(N)cc2)[C@@H]1O)O)/C1=*(CC)c2c(C1(C)C)cc(S(=O)(=O)[O-])cc2

Molecular Weight

1115.11 g/mol (free acid)

Molecular Formula

C46H64N6O18P2S2 (free acid)