Catalogue Number: NU-1706-CY3-JEN
Manufacturer: | Jena Bioscience |
Shelf Life: | 12 months |
Ph: | 7.5 +/-0 |
Physical state: | Solution |
Type: | Labelled Nucleotides |
Shipping Condition: | Blue Ice |
Unit(s): | 20 ul |
Description: pCp-Cy3 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Please note: Protect the Dye-labeled pCp from exposure to light and carry out experimental procedures under low light conditions.
1.0-1.1 mM
Incorporation into RNA/miRNA by3'-End Labeling with T4 RNA Ligase[1]
Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Cy3, Triethylammonium salt
C(=CC=C/1N(c2c(C1(C)C)cc(cc2)S(=O)(=O)O)CCCCCC(=O)NCCCCCCOP(=O)(O[C@@H]1[C@@H](COP(=O)(O)O)O[C@@H](n2c(=O)nc(N)cc2)[C@@H]1O)O)/C1=*(CC)c2c(C1(C)C)cc(S(=O)(=O)[O-])cc2
1115.11 g/mol (free acid)
C46H64N6O18P2S2 (free acid)