pCp-Cy5
Catalogue Number: NU-1706-CY5-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 ul |
Description
Description: pCp-Cy5 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Please note: Protect the Dye-labeled pCp from exposure to light and carry out experimental procedures under low light conditions.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into RNA/miRNA by3'-End Labeling with T4 RNA Ligase[1]
Short Description
Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Cy5, Triethylammonium salt
Smile String
C(=CC1=*(CC)c2c(C1(C)C)cc(S(=O)(=O)[O-])cc2)/C=C/C=C1/N(c2c(C1(C)C)cc(cc2)S(=O)(=O)O)CCCCCC(=O)NCCCCCCOP(=O)(O[C@@H]1[C@@H](COP(=O)(O)O)O[C@@H](n2c(=O)nc(N)cc2)[C@@H]1O)O
Molecular Weight
1141.14 g/mol (free acid)
Molecular Formula
C48H66N6O18P2S2 (free acid)
NU-1706-CY5 MSDS
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