Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) Antibody
Catalogue Number: P1311-53-PPS
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P1311-53 Image
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
P1311-53 Image
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
P1311-53 Image
Here is the legend: Immunostaining of mouse kidney sections from control (CTL) and BRL37344 treated (BRL, 1 mg/kg) specifically labeling localized phosphorylated NCC thr53 (Cat p1311-53, green) and counterstained with Evans Blue (in red). Image from publication. CC-BY-4.0, PMID: 38652212
P1311-53 Image
Two Western blots show the abundance of pNCCT53 (cat. p1311-53, 1:200) and tNCC in male Kcnj10fl/fl mice and in Ks-Kir4.1-KO mice treated with vehicle (control) and FK506 (0.75 mg/kg BW). Image from publication CC-BY-4.0. PMID: 36821372
P1311-53 Image
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
P1311-53 Image
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (I>>-Ptase, 1200 units for 30 min).
| Manufacturer: | Phosphosolutions |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH). |
| Application: | WB, IHC |
Additional Text
Purification
Affinity Purified
Molecular Weight
160
Modification
Phosphorylated, Thr53
Antibody Clonality
Polyclonal
Storage Note
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Short Description
Our Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NCC (Thiazide sensitive NaCl cotransporter) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Specificity
FLJ96318 antibody, Na Cl cotransporter antibody, Na Cl symporter antibody, Na-Cl symporter antibody, NaCl electroneutral thiazide sensitive cotransporter antibody, NCCT antibody, SLC12A3 antibody, S12A3_HUMAN antibody, slc12a3 antibody, Solute carrier family 12 (sodium/chloride transporters) member 3 antibody, Solute carrier family 12 member 3 antibody, Thiazide sensitive Na Cl cotransporter antibody, Thiazide sensitive sodium chloride cotransporter antibody, Thiazide-sensitive sodium-chloride cotransporter antibody, TSC antibody
Uniprot ID
P55017, P59158
Gene ID
20497; 6559
Gene Name
Slc12a3, SLC12A3
p1311-53 Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations