Anti-cdc2 (Tyr15) Antibody
Catalogue Number: P135-15-PPS
P135-15 Image
P135-15 Image
P135-15 Image
Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase, λ-Ptase (1200 units for 30 min).
P135-15 Image
Western blot of human T47D cells showing specific immunolabeling of the ~34 kDa cdc2 phosphorylated at Tyr15 (Control, Lane 1). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). Phosphospecificity is shown in lanes 3 and 4, where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase, I>>-Ptase (1200 units for 30 min).
| Manufacturer: | Phosphosolutions |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr15 of rat cdc2, conjugated to keyhole limpet hemocyanin (KLH). |
| Application: | WB |
Additional Text
Purification
Affinity Purified
Modification
Phosphorylated, Tyr15
Antibody Clonality
Polyclonal
Molecular Weight
34
Storage Note
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Short Description
Our Anti-cdc2 (Tyr15) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and Xenopus cdc2 (Tyr15) and is antigen affinity purified from pooled serum. It is great for use in WB.
Specificity
Cdc 2 antibody, Cdc2 antibody, CDC28A antibody, CDK-1 antibody, CDK 1 antibody, CDK1 antibody, CDK1_HUMAN antibody, CDKN1 antibody, CELL CYCLE CONTROLLER CDC2 antibody, Cell division control protein 2 antibody, Cell division control protein 2 homolog antibody, Cell division cycle 2 G1 to S and G2 to M antibody, Cell division protein kinase 1 antibody, Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, Cyclin-dependent kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 Cdk1 antibody, p34 protein kinase antibody, P34CDC2 antibody
Uniprot ID
P39951, P49760
Gene ID
1196; 54237
Gene Name
Cdk1, CLK2
p135-15 Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations