Anti-ERK/MAPK (Thr202/Tyr204) Antibody
Catalogue Number: P160-2024-PPS
P160-2024 Image
P160-2024 Image
P160-2024 Image
P160-2024 Image
P160-2024 Image
P160-2024 Image
P160-2024 Image
P160-2024 Image
P160-2024 Image
Immunolabeling of cultured mouse hippocampal neurons fixed and stained with anti-phospho-ERK/MAPK Thr202/Tyr204 (p160-2024, green, 1:100) and red nuclear stain Propidium Iodide. The labeling identifies an increase in ERK/MAPK phosphorylation when hippocampal neurons are treated with a specific ASIC1a activator, MitTx toxin (20 nM, 4 min). Image kindly provided by Carina Weissmann, IFIBYNE-CONICET.
P160-2024 Image
Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
P160-2024 Image
Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.
P160-2024 Image
Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr202/Tyr204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
P160-2024 Image
Immunolabeling of cultured mouse hippocampal neurons fixed and stained with anti-phospho-ERK/MAPK Thr202/Tyr204 (p160-2024, green, 1:100) and red nuclear stain Propidium Iodide. The labeling identifies an increase in ERK/MAPK phosphorylation when hippocampal neurons are treated with a specific ASIC1a activator, MitTx toxin (20 nM, 4 min). Image kindly provided by Carina Weissmann, IFIBYNE-CONICET.
P160-2024 Image
Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
P160-2024 Image
Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.
P160-2024 Image
Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr202/Tyr204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (I>>-Ptase, 1200 units for 30 min).
| Manufacturer: | Phosphosolutions |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK conjugated to keyhole limpet hemocyanin (KLH). |
| Application: | ICC, WB, IHC |
Additional Text
Purification
Affinity Purified
Molecular Weight
42/44
Modification
Phosphorylated, Thr202
Antibody Clonality
Polyclonal
Storage Note
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Short Description
Our Anti-ERK/MAPK (Thr202/Tyr204) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK/MAPK (Thr202/Tyr204) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Specificity
ERK 1 antibody, ERK 2 antibody, ERK-2 antibody, ERK1 antibody, erk1/2 antibody, ERK2 antibody, ERT1 antibody, ERT2 antibody, Extracellular signal regulated kinase 1 antibody, Extracellular signal-regulated kinase 2 antibody, MAP kinase 1 antibody, MAP kinase 2 antibody, MAP kinase isoform p42 antibody, MAP kinase isoform p44 antibody, MAPK 1 antibody, MAPK 2 antibody, MAPK 3 antibody, Mapk1 antibody, MAPK2 antibody, MAPK3 antibody, Mitogen-activated protein kinase 1 antibody, Mitogen-activated protein kinase 2 antibody, MK01_HUMAN antibody, p38 antibody, p40 antibody, p41 antibody, p42-MAPK antibody, PRKM 2 antibody
Uniprot ID
P28482, P63086
Gene ID
116590; 5594
Gene Name
Mapk1, MAPK1
p160-2024 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations