Blue Gel Loading Buffer, Loading buffer for agarose or polyacrylamide gels
Catalogue Number: PCR-254-BL-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | Sample Loading Buffer |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 5 x 1.8 ml |
| Application: | PCR |
Description
Description: Jena Bioscience Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels. The buffers contain tracking dyes as indicator for DNA fragment migration. In addition, they contain glycerol to add density and EDTA to inhibit nuclease activities. The buffers are optimized for loading of DNA fragments in a size range of:Cat. No.||Gel Loading Buffer||fragment size PCR-254-bl||Blue Gel Loading Buffer||larger than 500 bp PCR-254-gr||Green Gel Loading Buffer||from 100 to 2000 bp PCR-254-or||Orange Gel Loading Buffer||smaller than 500 bp 6x Gel Loading Buffer: 60 mM Tris-HCl (pH 7.5), 60 mM EDTA, 50 % (w/v) Glycerol, bromophenol blue (blue buffer), xylene cyanol FF (blue and green buffer), orange G (orange buffer) and tartrazine (green buffer) DNA sample||5 µl||10 µl||20 µl||50 µl 6x Gel Loading Buffer||1 µl||2 µl||4 µl||10 µl
Additional Text
Application Notes
Add one part of Gel Loading Buffer to 5 parts of the DNA sample as shown in the table below. Spin down the tubes and vortex gently to achieve homogeneity.DNA sample||5 µl||10 µl||20 µl||50 µl 6x Gel Loading Buffer||1 µl||2 µl||4 µl||10 µl
PCR-254-BL MSDS
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