qPCR GreenMaster UNG, Master mix for real-time qPCR with green-fluorescent DNA stain, 2 x conc. master mix
Catalogue Number: PCR-375S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | qPCR Mixes |
| Shipping Condition: | Blue Ice |
| Unit(s): | 2 x 1.25 ml |
| Application: | PCR |
Description
Description: qPCR GreenMaster UNG is designed for quantitative real-time analysis of DNA samples. The mix contains all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. It is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds. The mix is based on an optimized hot-start polymerase. Its activity is blocked by antibody at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. The green-fluorescent DNA stain intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the use of additional sequence-specific labeled probes (i.g. TaqMan® Probes). The mix contains UNG (Uracil-N-Glycosylase) and dUTP instead of dTTP to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. The mix can also be combined with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal. Green-fluorescent DNA stain Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling. Green-fluorescent DNA stain is not recommended for high-resolution melting curve analysis (HRM). To perform the assay, simply select the optical setting for SYBR® GREEN on the detection instrument.
Additional Text
Storage Note
store at -20 °C|12 months|avoid freeze/thaw cycles, store dark|Storage at 4 °C for up to 3 months possible.
PCR-375S Datasheet
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