SCRIPT Direct RT-qPCR GreenMaster, Robust real-time RT-PCR master mix with green-fluorescent DNA stain, for highly sensitive and specific amplification directly from tissue, swabs or blood
Catalogue Number: PCR-532S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Physical state: | Liquid |
| Type: | qPCR Mixes |
| Shipping Condition: | Blue Ice |
| Unit(s): | 2 x 1.25 ml |
| Application: | PCR |
Description
Description: SCRIPT Direct RT-qPCR GreenMaster is designed for quantitative real-time analysis of target RNA directly from animal- or plant tissue, swabs and blood. The mix allows robust amplification avoiding the requirement of any prior RNA purification procedures based on a green-fluorescent DNA stain that is structurally similar to SYBR® GREEN. It allows fast and easy quantification of sample RNA over a wide dynamic range with exceptional sensitivity and precision. The mix contains all reagents required for RT-qPCR (except template and primers) in a premixed 2 x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a genetically engineered reverse transcriptase and an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system. The mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for: Direct amplification of target RNA from various tissues samplesDirect detection of viral or bacterial RNA in nasal or throat swabsDirect PCR from whole samplesPoint-of-Care diagnostics. Green-fluorescent DNA stain Green-fluorescent DNA stain is structurally similar to SYBR® GREEN and a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable, providing convenience during routine handling. Green-fluorescent DNA stain is not recommended for high-resolution melting curve analysis (HRM). To perform the assay, simply select the optical setting for SYBR® GREEN on the detection instrument. ROX Reference Dye The mix can also be used in combination with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.
Additional Text
Concentration
2 X
Storage Note
store at -20 °C|12 months|avoid freeze/thaw cycles, store dark|stable at 4 °C for up to 4 weeks
PCR-532S Datasheet
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