Cynomolgus PLAU/uPA Protein (active form) (PLA-CM101)
Catalogue Number: PLA-CM101-KAT
PLA-CM101 Image
PLA-CM101 Image
PLA-CM101 Image
PLA-CM101 Image
PLA-CM101 Image
Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).
PLA-CM101 Image
Immobilized Cynomolgus PLAU, His Tag at 1µg/ml (100µl/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 25.1ng/ml determined by ELISA.
PLA-CM101 Image
Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/µg (QC Test).
PLA-CM101 Image
The purity of Cynomolgus PLAU (active form) is greater than 95% as determined by SEC-HPLC.
| Manufacturer: | Kactus |
| Shelf Life: | 12 months |
| Molecular Weight: | The protein has a predicted MW of 47.23 kDa. Due to protein lysis and glycosylation, the protein migrates to 23-25 kDa (long chain A), 35-40 kDa (chain B) and 50-60 kDa (long chain A&chain B) based on Tris-Bis PAGE result. |
| Physical state: | Lyophilized |
| Type: | Proteases |
| Host Cell: | HEK293 cells |
| Shipping Condition: | RT |
| Unit(s): | 100 ug, 500 ug |
| Application: | ELISA, WB |
Description
Description: Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Additional Text
Gene Name
PLAU
Protein Tag
HIS Tag
Uniprot ID
A0A2K5WND1
Gene ID
102135886
Short Description
Recombinant Cynomolgus PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu430, which consists of two chains: Long chain A and chain B. The long chain A is further cleaved to yield a short chain A and N-Terminus fragment.
Storage Note
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
PLA-CM101 Datasheet
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