HighYield T7 Azide RNA Labeling Kit, Preparation of randomly Azide-modified RNA probes by in vitro transcription with 5-Azido-C3-UTP
Catalogue Number: RNT-101-AZ-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | Nucleic Acid Labeling Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 reactions |
Description
Description: HighYield T7 Azide RNA Labeling Kit is designed to produce randomly Azide-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization, Northern Blot or crosslinking experiments. 5-Azido-C3-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % 5-Azido-C3-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of 5-Azido-C3-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Azide-modified RNA probe can subsequently be labeled by copper-free Click Chemistry with Dibenzocyclooctyne (DBCO)-functionalized detection reagents (e.g DBCO-functionalized fluorescent dyes or (Desthio)Biotin) thereby forming a stable, covalently labeled RNA probe[1]. The kit contains sufficient reagents for 25 labeling reactions of 20 µl each (35 % 5-Azido-C3-UTP substitution, 1 mM ATP, GTP, CTP, 0.65 mM UTP, 0.35 mM 5-Azido-C3-UTP).
Additional Text
Smile String
C(CCc1cn(c(=O)[nH]c1=O)[C@H]1[C@@H]([C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N=[N+]=[N-]
RNT-101-AZ MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations