HighYield T7 Cy3 RNA Labeling Kit, Preparation of randomly Cy3-modified RNA probes by in vitro transcription with UTP-X-CY3
Catalogue Number: RNT-101-CY3-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | Nucleic Acid Labeling Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 reactions |
Description
Description: HighYield T7 Cy3 RNA Labeling Kit is designed to produce randomly Cy3-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. UTP-X-CY3 (also known as Cy3-17-UTP from GE Healthcare) is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % UTP-X-Cy3 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-X-Cy3/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Cy3-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35 % UTP-X-Cy3 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-X-Cy3).
Additional Text
Smile String
P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H]([C@@H]([C@@H]1O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=CC=CC1=[N+](c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC
RNT-101-CY3 MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations