HighYield T7 Cy5 RNA Labeling Kit, Preparation of randomly Cy5-modified RNA probes by in vitro transcription with UTP-X-CY5
Catalogue Number: RNT-101-CY5-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | Nucleic Acid Labeling Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 20 reactions |
Description
Description: HighYield T7 Cy5 RNA Labeling Kit is designed to produce randomly Cy5-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. UTP-X-CY5 (also known as Cy5-17-UTP from GE Healthcare) is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % UTP-X-Cy5 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-X-Cy5/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Cy5-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35 % UTP-X-Cy5 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-X-Cy5).
Additional Text
Smile String
C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1[C@@H]([C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)NC(=O)CCCCCNC(=O)CCCCCN1/C(=C/C=C/C=C/C2=[N+](CC)c3c(C2(C)C)cc(S(=O)(=O)[O-])cc3)/C(c2c1ccc(c2)S(=O)(=O)O)(C)C
RNT-101-CY5 MSDS
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations