HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/ψ-UTP), Synthesis of ARCA-capped, 5-methylcytidine & pseudouridine-modified (m)RNA
Catalogue Number: RNT-103-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | mRNA Synthesis kit |
| Shipping Condition: | Blue Ice |
| Unit(s): | 15 rxns x 20ul |
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Description
Description: HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/ψ-UTP) is designed to produce large amounts of ARCA-capped, 5-methylcytidine and pseudouridine-modified (m)RNA via in vitro transcription with T7 RNA polymerase. The resulting 5'-capped and internally modified (m)RNA can subsequently be used for microinjection, transfection or in vitro translation experiments. Anti-reverse cap analog (ARCA, m7,3'-OGpppG)-capped (m)RNA possesses a significantly higher translation efficiency compared to traditional m7GpppG-capped (m)RNA. This is due to 3'-O-methylation of the m7 guanine moiety that allows ARCA incorporation in the correct ("anti-reverse") orientation only resulting in a 100 % translatable capped (m)RNA population. 5-Methylcytidine and pseudouridine modifications have been shown to increase (m)RNA stability and to reduce immunogenicity. The kit contains sufficient reagents for 15 labeling reactions a 20 µl (6 mM ARCA, 1.5 mM GTP, 7.5 mM 5-Methyl-CTP, 7.5 mM Pseudo-UTP, 7.5 mM ATP). An individual optimization of ARCA, 5-methyl-CTP and Pseudo-UTP concentration can easily be achieved with the single nucleotide format. A 20 µl reaction yields about 30-50 µg RNA after 30 min incubation (1 µg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation)).
Additional Text
Storage Note
store at -20 °C|12 months after date of delivery|avoid freeze/thaw cycles
RNT-103 MSDS
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