HighYield T73M Aptamer Synthesis Kit (2'F-dGTP), Synthesis of 2'-Fluoro-modified RNA

Catalogue Number: RNT-303-JEN

Manufacturer:Jena Bioscience
Shelf Life:12 months
Type:Nucleic Acid Labeling Kits
Shipping Condition:Blue Ice
Unit(s): 30 reactions

Description

Description: HighYield T73M Aptamer Synthesis Kit (2'F-dGTP) is designed to produce large amounts of 2'-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2'-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA polymerase (T73M RNA polymerase) ensures the efficient incorporation of 2'-fluoro modified dGTP instead of is natural counterpart GTP. The kit contains sufficient reagents for 30 reactions a 20 µl (7.5 mM ATP, 7.5 mM CTP, 7.5 mM 2'-Fluoro-dGTP, 7.5 mM UTP). An individual optimization of 2'-Fluoro-dGTP concentration can easily be achieved with the single nucleotide format. A 20 µl reaction yields about 30 µg RNA after 30 min incubation (1 µg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).