Description
Description: Chitozen is a chitosan-coated coverslip that can be used to image bacteria still or alive. It can also be used to perform long-term imaging or testing changes in growth conditions and analysing cell behaviour.
It is compatible with 6-channel sticky slides for flow experiments. It is very helpful if you want to:
Image bacteria both still and alive under the microscope
-Maintain bacteria in a same focal plane for imaging while preserving their physiology
-Renew culture medium, change growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly -observe, in real-time, the bacteria new comportment under the microscope
1 slide and 1 sticky slide per pack
Applications
Assay compatibility:
> Fluorescence
> Co-cultures (bacterial predators, immune cells)
> Addition of external factors (e.g. antibiotics, chemicals, inhibitors)
> Static or dynamic conditions
Imaging modes:
> phase-contrast,
> epifluorescence,
> confocal,
> super-resolution microscopy,
> atomic force microscopy (AFM) - documentation & example pictures available on request
Experimental outputs:
> Behavioural changes: growth, elongation, cell division, fitness, colony/biofilm formation, etc
> Single molecule imaging
Chitozen is efficient with the following bacteria:
> E. coli
> Bacillus subtilis
> Caulobacter crescentus
> Corynebacterium glutamicum
> Helicobacter pylori
> Mycobacterium smegmatis
> Myxococcus xanthus
> Pseudomonas aeruginosa
> Pseudomonas fluorescens
> Salmonella
> Staphylococcus aureus
> Vibrio cholerae
Technical specifications
Material: glass
Format: 75.0 x 25.0 mm
Thickness: 0.170 mm / 1.5H
Lifetime: up to 12 months at room temperature, shielded from direct sunlight
Additional Text
Short Description
Functionalized microscope coverslips for bacteria live imaging