Description
Description: Chitozen is a chitosan-coated coverslip immobilizing bacteria for microscopy without inducing bacteriostatic effects. It is compatible with 6-channel sticky slides for flow experiments. It is very helpful if you want to:
-Image bacteria both still and alive under the microscope
-Maintain bacteria in a same focal plane for imaging while preserving their physiology
-Renew culture medium, change growth condition (e.g. antibiotics, chemicals, inhibitors) during the experiment and directly observe, in real-time, the bacteria new comportment under the microscope
Kit contents
Chitozen coverslips are compatible with 6-channel sticky slides to work in a closed system in static or dynamic flow conditions. Order you coverslips alone if you need to work in an open system (i.e. for AFM imaging) or to place them on depression slides.
5-coverslips with microfluidic channels (= 30 experiments)
> 5x standard (25 x 75 mm) chitosan-coated coverslips
> 5x bottomless 6-channel sticky slides
Centrifugation pack: the Chitozen coverslips can be centrifuged using a rack compatible with standard microscope slide (25mmx75mm) dimensions. It includes:
> µ-Slide Microscopy Rack (ibidi
> Magnetic Lid for Microscopy Rack
> Clamp & adapter for sticky slides
Applications
Assay compatibility:
> Fluorescence
> Co-cultures (bacterial predators, immune cells)
> Addition of external factors (e.g. antibiotics, chemicals, inhibitors)
> Static or dynamic conditions
Imaging modes:
> phase-contrast,
> epifluorescence,
> confocal,
> super-resolution microscopy,
> atomic force microscopy (AFM) - documentation & example pictures available on request
Experimental outputs:
> Behavioural changes: growth, elongation, cell division, fitness, colony/biofilm formation, etc
> Single molecule imaging
Chitozen is efficient with the following bacteria:
> E. coli
> Bacillus subtilis
> Caulobacter crescentus
> Corynebacterium glutamicum
> Helicobacter pylori
> Mycobacterium smegmatis
> Myxococcus xanthus
> Pseudomonas aeruginosa
> Pseudomonas fluorescens
> Salmonella
> Staphylococcus aureus
> Vibrio cholerae
Technical specifications
Material: glass
Format: 75.0 x 25.0 mm
Thickness: 0.170 mm / 1.5H
Lifetime: up to 12 months at room temperature, shielded from direct sunlight
Additional Text
Short Description
Functionalized microscope coverslips for bacteria live imaging