Caspase 3 Polyclonal Antibody
Catalogue Number: BS-0081R-BSS
bs-0081R Image
bs-0081R Image
bs-0081R Image
bs-0081R Image
bs-0081R Image
Lane 1: Mouse Lung lysates; Lane 2: Human Hela cell lysates; Lane 3: Mouse NIH/3T3 cell lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Human Raji cell lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Mouse Lung lysates; Lane 4: Rat Lung lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
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Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Cerebrum lysates; Lane 5: Mouse NIH/3T3 cell lysates; Lane 6: Rat Spleen lysates; Lane 7: Rat Lung lysates; Lane 8: Rat Lymph node lysates; Lane 9: Rat Cerebrum lysates; Lane 10: Human Hela cell lysates probed with Caspase 3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bs-0081R Image
Rat lung lysate 30ug, probed (bs-0081R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CPP32; SCA-1; CPP32B; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; CASP3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Caspase 3(Active) |
| Application: | ICC, IF, WB |
Description
Description: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Additional Text
Gene Name
CASP3
Gene ID
836
Uniprot ID
P42574
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0081R Datasheet
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