AKT1/2/3 (Ser472/Ser473/Ser474) Antibody
Catalogue Number: BS-0876R-BSS
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bs-0876R Image
bs-0876R Image
bs-0876R Image
bs-0876R Image
Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-phospho-AKT/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Formalin-fixed and paraffin embedded mouse tumor with labeled Anti-phospho-AKT/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
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Mouse liver lysates probed with Rabbit Anti-AKT(Ser473) Polyclonal Antibody, Unconjugated (bs-0876) at 1:300 overnight at 4°C. Incubate with HRP conjugated Goat-Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000 for 90min at 37°C.
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U937 cell lysates probed with Anti-AKT1/2/3 (Ser472/Ser473/Ser474) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Akt; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; RAC-PK-alpha; Akt1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Ser473 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Additional Text
Gene Name
AKT1
Uniprot ID
P47196
Gene ID
24185
Modification
Phosphorylated, Ser473
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-0876R Datasheet
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