Beclin 1 Polyclonal Antibody
Catalogue Number: BS-1353R-BSS
bs-1353R Image
bs-1353R Image
bs-1353R Image
bs-1353R Image
bs-1353R Image
bs-1353R Image
bs-1353R Image
bs-1353R Image
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Beclin 1 Polyclonal Antibody (bs-1353R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
bs-1353R Image
Image provided by One World Lab validation program. SMMC7721 cells probed with Rabbit Anti-Beclin 1 Polyclonal Antibody (bs-1353R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
bs-1353R Image
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Beclin 1 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:400 overnight at 4°C, DAB staining.
bs-1353R Image
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (bs-1353R ) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
bs-1353R Image
Lane 1: mouse brain lysates Lane 2: mouse intestine lysates probed with Anti BECN1/Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 50kD.
bs-1353R Image
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (bs-1353R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
bs-1353R Image
Formalin-fixed and paraffin-embedded rat stomach labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ATG6; VPS3; beclin1; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human Beclin 1 |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Additional Text
Gene Name
BECN1
Gene ID
8678
Uniprot ID
Q14457
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Modification
Unmodified
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-1353R Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations