c-Raf(Tyr341) Polyclonal Antibody
Catalogue Number: BS-5650R-BSS
bs-5650R Image
bs-5650R Image
bs-5650R Image
Lane 1: A549 cell lysates; Lane 2: Lovo cell lysates probed with c-Raf(Tyr341) Polyclonal Antibody, Unconjugated (bs-5650R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
bs-5650R Image
Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti phospho-c-Raf(Tyr341) Polyclonal Antibody, Unconjugated (bs-5650R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; RAF1; RAF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Tyr341 |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Additional Text
Gene ID
5894
Gene Name
RAF1
Uniprot ID
P04049
Modification
Phosphorylated, Tyr341
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-5650R Datasheet
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