LC3 Polyclonal Antibody
Catalogue Number: BS-8878R-BSS
bs-8878R Image
bs-8878R Image
bs-8878R Image
bs-8878R Image
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:200 overnight at 4°C, DAB staining.
bs-8878R Image
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:200 followed by conjugation to the secondary antibody (bs-0295G-HRP) and DAB staining.
bs-8878R Image
Lane 1: mouse brain lysates probed with Rabbit Anti-LC3 Polyclonal Antibody, Unconjugated (bs-8878R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 20 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human LC3 |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Additional Text
Purification
Protein A purified
Concentration
1 ug/ul
Antibody Clonality
Polyclonal
Uniprot ID
Q9GZQ8, Q9H492
Gene ID
81631; 84557
Gene Name
MAP1LC3A, MAP1LC3B
Modification
Unmodified
Specificity
LC3A: Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha Microtubule-associated proteins 1A/1B light chain 3B LC3B: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta
Storage Note
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
bs-8878R Datasheet
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